The mIRage-LS IR multimodal microscope integrates the combined technologies of Raman and Fluorescence microscopy with sub-micron IR spatial resolution imaging and spectroscopy using Optical Photothermal IR (O-PTIR) spectroscopy. These combined techniques will enable life science researchers to easily identify bio-molecular structures of interest and characterize their chemical structure with submicron IR.
Sub-micron infrared and simultaneous Raman spectroscopy | ||||
Technique | Spatial resolution | Spectral range | Mode | Probe laser (O-PTIR/Raman) |
O-PTIR | < 500 nm | 1800-800 cm-1 3600-2700 cm-1 |
Reflection Transmission Counter-propagating |
532 nm 785 nm |
Raman | < 500 nm | 3900-200 cm-1 | Reflection |
Widefield Epi Fluorescence | |||
Filter cubes | Illumination | Camera | Objectives |
Standard filter cubes available | Combined 6 solid state white lights with liquid light guide | Sensor: back illuminated sCMOS monochrome Resolution: 2048 x 2048 Quantum Efficiency: up to 95% Spectral Range: 370 – 1100 nm |
Standard optical objectives available |
1. Co-located Fluorescence microscopy and sub-micron IR spectroscopy
2. <500nm IR and Raman spatial resolution
3. Simultaneous IR and Raman spectroscopy
4. Non-contact reflection-based IR measurement with FTIR transmission-like spectral quality
5. No IR spectral artifacts like Mie/diffuse scattering or specular reflection
6. Hydrated cell imaging (IR, Raman and Fluorescence)
(A) Life science-cells
(B) Life science-tissue
(C) Life science-bacteria
(D) Microplastics
(E) Polymers